Prof. Fernanda Tovar-Moll, MD, PhD
Brain Connectivity Unit
D'Or Institute for Research and Education and
Federal University of Rio de Janeiro
 | (A) Cytotoxicity of mitomycin C (MMC). Phase-contrast
micrographs showing the general morphology of control (A1) and MMC-treated
mESC colonies (A2--4). Cell death induced by treatment with increasing MMC
concentrations for 12 h (A5) was measured by flow cytometry as the
percentage of caspase 3 positive cells. At 1 µg/mL, the effect of MMC was not
significant (n = 3). (B) Assessment of neural differentiation in vitro: after 14 days
of co-culture onto meningeal cells, most GFP-mESCs (green) pre- |
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 Tumorigenicity assay in nude mice ...(A) Photograph of the hindlimbs from a nude mouse 12
weeks after intramuscular injection of 500,000 mitomycin-treated (right) and
untreated (left) mESCs. In all six animals tested, the right limbs were normal and
the left limbs displayed large tumors. (B) In vivo MRI scans, coronal plane,
proton density sequence, showing teratoma formation in the left limb, as
opposed to the right limb (which received mitomycin-treated mESCs) of the
same mouse. (C) In vivo MRI scans, axial plane, T1-weighted seq |
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 Transplantation of fully undiffer...(A) Survival curve of control and transplanted 6-OHDA-lesioned mice:
4 weeks after 6-OHDA injection in the striatum, mice were injected at the
same stereotaxic coordinates with culture medium (lesion-control, n = 8),
untreated mESCs (mESC, n = 8), or mitomycin-treated mESCs (MMCmESC,
n = 12). (B,C) In vivo brain MRI, coronal plane, T1-weighted
sequence, prior to (left-hand panel) and after (right-hand panels)
gadolinium injection evidencing striatal tumor 4 weeks after transplantation
of untreat |
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